Deliverable 4.1:
Combined Magnetic and Optical Tweezer Set-up
CNRS/ENS will produce both a magnetic tweezer and an optical
tweezer set-up for measuring the forces required for DNA
strand separation of the above palindromic DNA.
Outcomes:
We
have built a set-up that allows the simultaneous manipulation
of DNA with a magnetic tweezer and the visualization of a
single protein tagged with a fluorophore. That set-up needs
still to be tested and calibrated. It will then be used to
observe the action of a number of DNA translocases, among
which will be EcoR124I studied by the groups at Portsmouth and
Delft.
Project Outcomes
